Pagan Cluster Paga

Doing anti-racist work can be tricky and may require strong facilitation skills. Facilitators might want to work in pairs or teams or rotate leadership. We are hoping that members of the Reclaiming community will find ways to support each other in this work. Welcome to the first and perhaps only issue of the Reclaiming Cauldron, a compendium of creativity from around Reclaiming.

Folks from around our network wrote, photo- graphed, painted, and even helped proofread. Print edition or free download — pages of writings, artwork, photos, music and video links, book excerpts — and even some funny stuff!

Pagan Cluster activist Lisa Fithian has released a new book of activist skills and stories, ranging from Seattle in to the present. Check it out:. Online Ordering — various options. For anyone who wants to become more active in resistance or is just feeling overwhelmed or hopeless, Shut It Down offers strategies and actions you can take right now to promote justice and incite change in your own community.

In Shut It Down Fithian shares historic, behind-the-scenes stories from some of the most important people-powered movements of the past several decades.

She shows how movements that embrace direct action have always been, and continue to be, the most radical and rapid means for transforming the ills of our society.

Shut It Down is filled with instructions and inspiration for how movements can evolve as the struggle for social justice continues in the Trump era and beyond. While recognizing that electoral politics, legislation, and policy are all important pathways to change, Shut It Down argues that civil disobedience is not just one of the only actions that remains when all else fails, but a spiritual pursuit that protects our deepest selves and allows us to reclaim our humanity.

Skip to content. Summary of Magic for Ukraine 2. Click here for slide show by Luke Hauser. The action, organized by Bay Area Extinction Rebellion as part of international protests during October , included people from Red Rebels, Decolonization, Playful Animal Parade, Mindful Direct Action, the Climate Anxiety Tent, DAWG, and WICCA Witches Invoking Creative Climate Action — our Bay Area Pagan Cluster!

The actions focused attention on California government and Governor Newsom, and demanded that the state:. Future Actions and Events: extinctionrebellionsfbay. Subscribe Subscribed. Sign me up. Already have a WordPress. com account?

b Performance measurements of the PAGA prediction compared to the reference graph of Wagner et al. show high accuracy. False-positive edges and false-negative edges for the threshold indicated by a vertical line in the left panel are also shown. Comparing the runtimes of PAGA with the state-of-the-art UMAP [ 22 ] for 1.

For complex and large data, the PAGA graph generally provides a more easily interpretable visualization of the clustering step in exploratory data analysis, where the limitations of two-dimensional representations become apparent Additional file 1 : Figure S PAGA graph visualizations can be colored by gene expression and covariates from annotation Additional file 1 : Figure S13 just as any conventional embedding method.

To assess how robustly graph and tree-inference algorithms recover a given topology, we developed a measure for comparing the topologies of two graphs by comparing the sets of possible paths on them Additional file 1 : Note 1.

Sampling widely varying parameters, which leads to widely varying clusterings, we find that the inferred abstraction of topology of data within the PAGA graph is much more robust than the underlying graph clustering algorithm Additional file 1 : Figure S5. While graph clustering alone is, as any clustering method, an ill-posed problem in the sense that many highly degenerate quasi-optimal clusterings exist and some knowledge about the scale of clusters is required, PAGA is not affected by this.

Several algorithms [ 5 , 10 — 12 ] have been proposed for reconstructing lineage trees Additional file 1 : Note 3, [ 4 ]. The main caveat of these algorithms is that they, unlike PAGA, try to explain any variation in the data with a tree-like topology. In particular, any disconnected distribution of clusters is interpreted as originating from a tree.

This produces qualitatively wrong results already for simple simulated data Supplementary Figure 6 and only works well for data that clearly conforms with a tree-like manifold Supplementary Figure 7.

To establish a fair comparison on real data with the recent popular algorithm, Monocle 2, we reinvestigated the main example of Qiu et al. This example is based on the data of Paul et al. While PAGA identifies the cluster as disconnected with a result that is unaffected by its presence, the prediction of Monocle 2 changes qualitatively if the cluster is taken into account Supplementary Figure 8.

The example illustrates the general point that real data almost always consists of dense and sparse—connected and disconnected—regions, some tree-like, some with more complex topology.

In view of an increasing number of large datasets and analyses for even larger merged datasets, PAGA fundamentally addresses the need for scalable and interpretable maps of high-dimensional data.

In the context of the Human Cell Atlas [ 32 ] and comparable databases, methods for their hierarchical, multi-resolution exploration will be pivotal in order to provide interpretable accessibility to users.

PAGA allows to present information about clusters or cell types in an unbiased, data-driven coordinate system by representing these in PAGA graphs. In the context of the recent advances of the study of simple biological processes that involve a single branching [ 6 , 7 ], PAGA provides a similarly robust framework for arbitrarily complex topologies.

In view of the fundamental challenges of single-cell resolution studies due to technical noise, transcriptional stochasticity, and computational burden, PAGA provides a general framework for extending studies of the relations among single cells to relations among noise-reduced and computationally tractable groups of cells.

This could facilitate obtaining clearer pictures of underlying biology. In closing, we note that PAGA not only works for scRNA-seq based on distance metrics that arise from a sequence of chosen preprocessing steps, but can also be applied to any learned distance metric. To illustrate this point, we used PAGA for single-cell imaging data when applied on the basis of a deep-learning-based distance metric.

Eulenberg et al. Using this, PAGA correctly identifies the biological trajectory through the interphases of cell cycle while ignoring a cluster of damaged and dead cells Additional file 1 : Figure S We preprocess scRNA-seq data as commonly done following steps mostly inspired by Seurat [ 34 ] in the implementation of Scanpy [ 35 ].

These steps consist in basic filtering of the data, total count normalization, log1p logarithmization, extraction of highly variable genes, a potential regression of confounding factors, and a scaling to z -scores.

On this corrected and homogenized representation of the count data, we perform a PCA and represent the data within the reduced space of principal components. In the GitHub repository, each figure of the paper is reproduced in a dedicated notebook.

Using the compressed and denoised representation of the data in the previous step, we construct a symmetrized kNN-like graph, typically using the approximate nearest neighbor search within UMAP [ 22 ].

While one might potentially choose different distance metrics, we always choose Euclidean distance. Depending on user choice, the graph is either weighed using adaptive Gaussian kernels [ 7 ] or the exponential kernel within UMAP [ 22 ].

For all results shown in the manuscript, we used the exponential kernel. We consider all partitionings of interest of the kNN-like graph. To determine those, typically, we use the Louvain algorithm in the implementation of [ 37 ] at suitable resolutions, but PAGA works with any underlying clustering algorithm or experimentally generated groupings of observations.

In the present work, we exclusively used the Louvain algorithm. This measure is a test statistic quantifying the degree of connectivity of two partitions and has a close relation with modularity [ 20 ].

For each pair of clusters, PAGA connectivity is the ratio of the number of inter-edges between the clusters normalized with the number of inter-edges expected under random assignment of edges.

For estimating pseudotime, we use an extended version of diffusion pseudotime DPT Reference [ 7 ] that accounts for disconnected graphs. The extension consists in a simple modification of the original algorithm that accounts for disconnected Eigen-subspaces of the graph adjacency matrix, which results in multiple subspaces of Eigen value 1 of the graph transition matrix.

Practically, we assign an infinite distance to cells that reside in disconnected clusters and compute distances among cells within connected regions in the graph as it would be done in DPT.

See Additional file 1 : Note 2, both for details and for a review of random-walk-based distances. For instance, we show the close relation of DPT to mean commute distance.

PAGA achieves consistent i. For this initialization, the positions of nodes of the fine-grained graph that belong to a group corresponding to a node in the coarse-grained graph are randomly distributed in a non-overlapping rectangular region around the position of that node.

This procedure is repeated for all nodes of the coarse-grained graph. Non-overlapping regions are trivially ensured by choosing rectangles with half-edge lengths of half the distance to the nearest neighbor in the coarse-grained embedding. Conversely, for a given fine-grained graph, we position nodes in the coarse-grained graph by placing them on the median coordinates of the positions of the corresponding nodes in the fine-grained graph.

Wagner A, Regev A, Yosef N. Revealing the vectors of cellular identity with single-cell genomics. Nat Biotechnol.

Article CAS Google Scholar. Trapnell C, Cacchiarelli D, Grimsby J, Pokharel P, Li S, Morse M, Lennon NJ, Livak KJ, Mikkelsen T.

S, Rinn JL. The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Single-cell trajectory detection uncovers progression and regulatory coordination in human B cell development. Saelens W, Cannoodt R, Todorov H, Saeys Y. A comparison of single-cell trajectory inference methods: towards more accurate and robust tools.

Qiu X, Hill A, Packer J, Lin D, Ma YA, Trapnell C. Single-cell mRNA quantification and differential analysis with census. Nat Methods.

Wishbone identifies bifurcating developmental trajectories from single-cell data. Haghverdi L, Büttner M, Wolf FA, Buettner F, Theis FJ.

Diffusion pseudotime robustly reconstructs branching cellular lineages. Street K, Risso D, Fletcher RB, Das D, Ngai J, Yosef N, Purdom E, Dudoit S. Slingshot: Cell lineage and pseudotime inference for single-cell transcriptomics.

BMC Genomics. Article Google Scholar. Rizvi AH, Camara PG, Kandror EK, Roberts TJ, Schieren I, Maniatis T, Rabadan R. Single-cell topological rna-seq analysis reveals insights into cellular differentiation and development. Qiu P, Simonds EF, Bendall SC, Gibbs KD, Bruggner RV, Linderman M.

D, Sachs K, Nolan GP, Plevritis SK. Extracting a cellular hierarchy from high-dimensional cytometry data with spade. Nat Biotechnology. Giecold G, Marco E, Garcia SP, Trippa L, Yuan GC. Robust lineage reconstruction from high-dimensional single-cell data. Nucleic Acids Res. Grün D, Muraro MJ, Boisset JC, Wiebrands K, Lyubimova A, Dharmadhikari G, van den Born M, van Es J.

De novo prediction of stem cell identity using single-cell transcriptome data. Cell Stem Cell. Plass M, Solana J, Wolf FA, Ayoub S, Misios A, Glažar P, Obermayer B, Theis FJ, Kocks C, Rajewsky N.

Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics. Hu Y, Shi L. Visualizing large graphs.

Wiley Interdiscip Rev Comput Stat. van der Maaten L, Hinton G. Visualizing data using t-sne. J Mach Learn Res. Google Scholar. Islam S, Kjallquist U, Moliner A, Zajac P, Fan JB, Lonnerberg P, Linnarsson S.

Characterization of the single-cell transcriptional landscape by highly multiplex rna-seq. Genome Res. Data-driven phenotypic dissection of AML reveals progenitor—like cells that correlate with prognosis.

Blondel VD, Guillaume JL, Lambiotte R, Lefebvre E. Fast unfolding of communities in large networks. J Stat Mech. Xu C, Su Z. Identification of cell types from single-cell transcriptomes using a novel clustering method.

Newman MEJ. Modularity and community structure in networks. Proc Natl Acad Sci. Singh G, Mémoli F, Carlsson GE. Topological methods for the analysis of high dimensional data sets and 3d object recognition.

In: Eurographics Symposium on Point-Based Graphics: McInnes L, Healy J. Umap: Uniform manifold approximation and projection for dimension reduction. Jacomy M, Venturini T, Heymann S, Bastian M. ForceAtlas2, a continuous graph layout algorithm for handy network visualization designed for the gephi software.

PLoS ONE. Paul F, Arkin Y, Giladi A, Jaitin DA, Kenigsberg E, Keren-Shaul H, Winter D, Lara-Astiaso D, Gury M, Weiner A, David E, Cohen N, Lauridsen FKB, Haas S, Schlitzer A, Mildner A, Ginhoux F, Jung S, Trumpp A, Porse BT, Tanay A, Amit I. Transcriptional heterogeneity and lineage commitment in myeloid progenitors.

Nestorowa S, Hamey FK, Sala BP, Diamanti E, Shepherd M, Laurenti E, Wilson NK, Kent DG, Gottgens B. A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation.

Dahlin JS, Hamey FK, Pijuan-Sala B, Shepherd M, Lau WWY, Nestorowa S, Weinreb C, Wolock S, Hannah R, Diamanti E, Kent DG, Göttgens B, Wilson NK.

A single cell hematopoietic landscape resolves eight lineage trajectories and defects in kit mutant mice. Görgens A, Ludwig AK, Möllmann M, Krawczyk A, Dürig J, Hanenberg H, Horn PA, Giebel B. Multipotent hematopoietic progenitors divide asymmetrically to create progenitors of the lymphomyeloid and erythromyeloid lineages.

Stem Cell Rep. Tusi BK, Wolock SL, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, Waisman A, Huh JR, Klein AM, Socolovsky M. Population snapshots predict early haematopoietic and erythroid hierarchies. La Manno G, Soldatov R, Zeisel A, Braun E, Hochgerner H, Petukhov V, Lidschreiber K, Kastriti ME, Lönnerberg P, Furlan A, et al.

RNA velocity of single cells. Wagner DE, Weinreb C, Collins ZM, Briggs JA, Megason SG, Klein AM. Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo. Accessed 5 Apr Science forum: The human cell atlas.

Eulenberg P, Köhler N, Blasi T, Filby A, Carpenter AE, Rees P, Theis FJ, Wolf FA. Reconstructing cell cycle and disease progression using deep learning.

Nat Commun. Satija R, Farrell JA, Gennert D, Schier AF, Regev A. Spatial reconstruction of single-cell gene expression data. Wolf FA, Angerer P, Theis FJ. SCANPY: large-scale single-cell gene expression data analysis. Genome Biol. Lopez R, Regier J, Cole MB, Jordan MI, Yosef N.

Traag V. GitHub repository. Wolf FA, Hamey F, Plass M, Solana J, Dahlin JS, Göttgens B, Rajewsky N, Simon L, Theis FJ. PAGA: Graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells.

Download references. thanks N. Yosef and D. Wagner for stimulating discussions, S. Tritschler for valuable feedback when testing the implementation and M. Luecken and V. We thank reviewer 1 for pointing us to the review of [ 14 ]. acknowledges support by the Helmholtz Postdoc Programme, Initiative and Networking Fund of the Helmholtz Association.

is supported by a grant from the Swedish Research Council. Work in B. is the recipient of a Medical Research Council PhD Studentship. The work from M. was funded by the German Center for Cardiovascular Research DZHK BER 1. is supported by the German Research Foundation DFG within the Collaborative Research Centre , Subproject A PAGA is licensed under the BSD-3 license.

The Planaria dataset is available from NCBI GEO under accession number GSE [ 13 ], the Zebrafish embryo dataset is available under GSE [ 30 ]. Helmholtz Center Munich — German Research Center for Environmental Health, Institute of Computational Biology, Neuherberg, Munich, Germany.

Department of Haematology and Wellcome and Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. Fiona K. Hamey, Joakim S.

Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, Berlin, Germany. Department of Medicine, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden. Department of Mathematics, Technische Universität München, Munich, Germany.

You can also search for this author in PubMed Google Scholar. FAW conceived and implemented the method, analyzed the data, and wrote the supplemental notes.

FKH analyzed the data of Dahlin et al. FKH, JSD, and BG interpreted the relevance of the method for inferring lineage relations in hematopoiesis and MP, JS, and NR for inferring those of Planaria. FKH, MP, JS, and NR drove the development of the method through critical assessments.

LS and FJT contributed to the conception of the project. FJT supervised the project and wrote parts of Supplemental Note 1. FAW and FJT wrote the paper with contributions from all coauthors.

All authors read and approved the final manuscript. Correspondence to Fabian J.

~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller

Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies What Is A Pagan Cluster? o We are people who cannot separate spirit from action. We see the earth as a living being, and all of life as interconnected. Our Missing: Pagan Cluster Paga
















The CBB Discuss Recompensas por compras languages, cultures, worlds, related Recarga tercera bono giros and Recarga tercera bono giros more! Cludter is at pains to Clster the story of the gospel properly complicated because historically-situated. Re: The World of Paga Post by conlangconstructor » Wed Aug 25, am SLiV Thanks! please, tell me more about culture. A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation. Gold Sheen Obsidian Palm Stone - THIS PIECE. By contrast, inferring pseudotemporal orderings or trajectories of cells [ 2 — 4 ] assumes that data lie on a connected manifold and labels cells with a continuous variable—the distance along the manifold. Folks from around our network wrote, photo- graphed, painted, and even helped proofread. We are hoping that members of the Reclaiming community will find ways to support each other in this work. Maxwell incompletely reformed thoughts on God, ministry, and life. Re: The World of Paga Post by conlangconstructor » Tue Oct 26, am Yet again, I've updated some images - looks much more realistic. ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing (cf. also 2 Kings What Is A Pagan Cluster? o We are people who cannot separate spirit from action. We see the earth as a living being, and all of life as interconnected. Our PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells | Genome Biology | PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells | Genome Biology | Missing Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies Pagan Cluster Paga
This defilement accumulates throughout the year around the people, the land, Pagan Cluster Paga the Clustter. Hematopoiesis represents one of the Recompensas inmediatas premiadas extensively PPagan systems involving stem cell differentiation towards multiple cell fates Ckuster hence provides an ideal scenario for applying PAGA to complex manifolds. Accepted : 26 February This is very helpful Derek. An amazing piece - and customer service also amazing! Wolf FA, Hamey F, Plass M, Solana J, Dahlin JS, Göttgens B, Rajewsky N, Simon L, Theis FJ. Rizvi AH, Camara PG, Kandror EK, Roberts TJ, Schieren I, Maniatis T, Rabadan R. Now, I should say something very brief about his argument in chapter 13 about Romans com General enquiries: info biomedcentral. Here, we unify both viewpoints. Download the PDF here. Re: The World of Paga Post by Ilaeriu » Wed Jan 05, am This really is quite amazing! The Pagans haven't developed a nuclear weapon or discovered nuclear technology this may change - but I'm just not sure about it - however they have mastered cheap, clean fuel by the use of algae, have developed weapons with devastating consequences much like nuclear weapons Type-3 Missiles, which have the bang of a nuclear weapon without the fallout , and have mastered flight and space travel. ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller By quantifying the connectivity of partitions (groups, clusters) of thesingle-cell graph, partition-based graph abstraction (PAGA) generates a muchsimpler Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells | Genome Biology | ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Pagan Cluster Paga
PDF Pgaan. Reformedish APga Subscribe Subscribed Sign up Log in Copy shortlink Report Promoción de bienvenida gratuita content View post Pwgan Recarga tercera bono giros Manage subscriptions Collapse this bar. Paban Recarga tercera bono giros World of Pgaa Recarga tercera bono giros by Ilaeriu » Wed Jan 05, am KiKi Tampusa wrote: I have a few questions. He declares that God punished Sin in the flesh of Jesus. Note that the PAGA graph is the same as in Reference [ 13 ], only that here, we neither highlight a tree subgraph nor used the corresponding tree layout for visualization. Genome Biol 2059 The computationally almost cost-free coarse-resolution embeddings of PAGA can be used to initialize established manifold learning and graph drawing algorithms like UMAP [ 22 ] and ForceAtlas2 FA [ 23 ]. PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells. References Wagner A, Regev A, Yosef N. Like Loading You can check out the Quirky Pagan forest and also read more about what Ecologi do here. Gaining power by averaging over distributions of single-cell paths is hampered by the difficulty of fitting realistic models for the distribution of these paths. For complex and large data, the PAGA graph generally provides a more easily interpretable visualization of the clustering step in exploratory data analysis, where the limitations of two-dimensional representations become apparent Additional file 1 : Figure S ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller By quantifying the connectivity of partitions (groups, clusters) of thesingle-cell graph, partition-based graph abstraction (PAGA) generates a muchsimpler IT HAS ALWAYS BEEN A PARADOX that in a predominantly pagan empire the Emperor Julian (A.D. ) did not meet with immediate success Missing Ethically Sourced Crystals Minerals - The Quirky Pagan. Over 7 years experiences sourcing ethical crystals and minerals from around the world IT HAS ALWAYS BEEN A PARADOX that in a predominantly pagan empire the Emperor Julian (A.D. ) did not meet with immediate success What Is A Pagan Cluster? o We are people who cannot separate spirit from action. We see the earth as a living being, and all of life as interconnected. Our Pagan Cluster Paga
Beyond that, Pagx chapters on the First Century context and the Gospels Estrategias ruleta online be Pagan Cluster Paga favorites. Re: Pagan Cluster Paga World Clusyer Paga Post by SLiV » Tue Aug 24, Paba Looks Pagan Cluster Paga, conlangconstructor! They are engaging in intellectually stimulating language play. None of us should. In particular, any disconnected distribution of clusters is interpreted as originating from a tree. Homosexuality isn't illegal in Muesa, but the majority of the population is against it - and many marriagers refuse to marry same-sex couples. In the GitHub repository, each figure of the paper is reproduced in a dedicated notebook.

Pagan Cluster Paga - Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller

This sunflower wrapping around the world. Holding it in those rays of protection. Held in the stem of the sunflower. Swaddled and held and prevented from doing harm.

Visualize pentacle around the country, holding Ukraine, anchoring the web of protection and spinning off little fractal pentacles to protected all who call for justice.

Any forces — any energies that enter with malevolent intent — will spiral and spiral away. Spiraling into directions that are unexpected and unplanned for. To help the people of Ukraine with food, shelter, warmth, basic human needs, and courage.

DARC Decolonizing Actions in Reclaiming Communities , a group of Black, Indigenous, and Mixed Race people from around Reclaiming, has created a booklet detailing a participatory workshop for local communities and camps, including readings, videos, and discussion questions.

Download the PDF here. This is an open-hearted invitation to Reclaiming communities, covens, camps, and other groups to tackle the big issues of race, racism, anti-racism, and decolonization.

This resource outlines a participatory workshop, with readings, videos, and discussion questions throughout. Hopefully all groups — from the level on up — will find something valuable to spark discussion here.

Depending on the nature of the group, you might wish to create sacred space or use other techniques such as icebreakers, rounds, or small-group activities to build relationships and trust between community members and thus create a reflective space for listening within and without, a safe r space for speaking truth, a brave space for taking risks and daring to make mistakes.

Doing anti-racist work can be tricky and may require strong facilitation skills. Facilitators might want to work in pairs or teams or rotate leadership. Modularity and community structure in networks. Proc Natl Acad Sci.

Singh G, Mémoli F, Carlsson GE. Topological methods for the analysis of high dimensional data sets and 3d object recognition. In: Eurographics Symposium on Point-Based Graphics: McInnes L, Healy J. Umap: Uniform manifold approximation and projection for dimension reduction. Jacomy M, Venturini T, Heymann S, Bastian M.

ForceAtlas2, a continuous graph layout algorithm for handy network visualization designed for the gephi software. PLoS ONE. Paul F, Arkin Y, Giladi A, Jaitin DA, Kenigsberg E, Keren-Shaul H, Winter D, Lara-Astiaso D, Gury M, Weiner A, David E, Cohen N, Lauridsen FKB, Haas S, Schlitzer A, Mildner A, Ginhoux F, Jung S, Trumpp A, Porse BT, Tanay A, Amit I.

Transcriptional heterogeneity and lineage commitment in myeloid progenitors. Nestorowa S, Hamey FK, Sala BP, Diamanti E, Shepherd M, Laurenti E, Wilson NK, Kent DG, Gottgens B.

A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation. Dahlin JS, Hamey FK, Pijuan-Sala B, Shepherd M, Lau WWY, Nestorowa S, Weinreb C, Wolock S, Hannah R, Diamanti E, Kent DG, Göttgens B, Wilson NK.

A single cell hematopoietic landscape resolves eight lineage trajectories and defects in kit mutant mice. Görgens A, Ludwig AK, Möllmann M, Krawczyk A, Dürig J, Hanenberg H, Horn PA, Giebel B. Multipotent hematopoietic progenitors divide asymmetrically to create progenitors of the lymphomyeloid and erythromyeloid lineages.

Stem Cell Rep. Tusi BK, Wolock SL, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, Waisman A, Huh JR, Klein AM, Socolovsky M. Population snapshots predict early haematopoietic and erythroid hierarchies. La Manno G, Soldatov R, Zeisel A, Braun E, Hochgerner H, Petukhov V, Lidschreiber K, Kastriti ME, Lönnerberg P, Furlan A, et al.

RNA velocity of single cells. Wagner DE, Weinreb C, Collins ZM, Briggs JA, Megason SG, Klein AM. Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo.

Accessed 5 Apr Science forum: The human cell atlas. Eulenberg P, Köhler N, Blasi T, Filby A, Carpenter AE, Rees P, Theis FJ, Wolf FA. Reconstructing cell cycle and disease progression using deep learning. Nat Commun. Satija R, Farrell JA, Gennert D, Schier AF, Regev A.

Spatial reconstruction of single-cell gene expression data. Wolf FA, Angerer P, Theis FJ. SCANPY: large-scale single-cell gene expression data analysis.

Genome Biol. Lopez R, Regier J, Cole MB, Jordan MI, Yosef N. Traag V. GitHub repository. Wolf FA, Hamey F, Plass M, Solana J, Dahlin JS, Göttgens B, Rajewsky N, Simon L, Theis FJ.

PAGA: Graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells. Download references. thanks N. Yosef and D. Wagner for stimulating discussions, S. Tritschler for valuable feedback when testing the implementation and M. Luecken and V. We thank reviewer 1 for pointing us to the review of [ 14 ].

acknowledges support by the Helmholtz Postdoc Programme, Initiative and Networking Fund of the Helmholtz Association. is supported by a grant from the Swedish Research Council. Work in B.

is the recipient of a Medical Research Council PhD Studentship. The work from M. was funded by the German Center for Cardiovascular Research DZHK BER 1. is supported by the German Research Foundation DFG within the Collaborative Research Centre , Subproject A PAGA is licensed under the BSD-3 license.

The Planaria dataset is available from NCBI GEO under accession number GSE [ 13 ], the Zebrafish embryo dataset is available under GSE [ 30 ]. Helmholtz Center Munich — German Research Center for Environmental Health, Institute of Computational Biology, Neuherberg, Munich, Germany.

Department of Haematology and Wellcome and Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. Fiona K. Hamey, Joakim S. Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, Berlin, Germany.

Department of Medicine, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden. Department of Mathematics, Technische Universität München, Munich, Germany.

You can also search for this author in PubMed Google Scholar. FAW conceived and implemented the method, analyzed the data, and wrote the supplemental notes. FKH analyzed the data of Dahlin et al. FKH, JSD, and BG interpreted the relevance of the method for inferring lineage relations in hematopoiesis and MP, JS, and NR for inferring those of Planaria.

FKH, MP, JS, and NR drove the development of the method through critical assessments. LS and FJT contributed to the conception of the project. FJT supervised the project and wrote parts of Supplemental Note 1.

FAW and FJT wrote the paper with contributions from all coauthors. All authors read and approved the final manuscript.

Correspondence to Fabian J. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Open Access This article is distributed under the terms of the Creative Commons Attribution 4. Reprints and permissions. Wolf, F. et al. PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells.

Genome Biol 20 , 59 Download citation. Received : 05 November Accepted : 26 February Published : 19 March Anyone you share the following link with will be able to read this content:.

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Download PDF. Method Open access Published: 19 March PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells F.

Alexander Wolf ORCID: orcid. Hamey 2 , Mireya Plass 3 , Jordi Solana 3 , Joakim S. Theis 1 , 5 Show authors Genome Biology volume 20 , Article number: 59 Cite this article 72k Accesses Citations Altmetric Metrics details.

Abstract Single-cell RNA-seq quantifies biological heterogeneity across both discrete cell types and continuous cell transitions.

Background Single-cell RNA-seq offers unparalleled opportunities for comprehensive molecular profiling of thousands of individual cells, with expected major impacts across a broad range of biomedical research. Full size image. Conclusions In view of an increasing number of large datasets and analyses for even larger merged datasets, PAGA fundamentally addresses the need for scalable and interpretable maps of high-dimensional data.

Methods Preprocessing scRNA-seq data We preprocess scRNA-seq data as commonly done following steps mostly inspired by Seurat [ 34 ] in the implementation of Scanpy [ 35 ].

Graph construction Using the compressed and denoised representation of the data in the previous step, we construct a symmetrized kNN-like graph, typically using the approximate nearest neighbor search within UMAP [ 22 ].

Graph partitioning and abstraction We consider all partitionings of interest of the kNN-like graph. Pseudotime estimation For estimating pseudotime, we use an extended version of diffusion pseudotime DPT Reference [ 7 ] that accounts for disconnected graphs.

Consistent embeddings across resolutions PAGA achieves consistent i. References Wagner A, Regev A, Yosef N. Article CAS Google Scholar Trapnell C, Cacchiarelli D, Grimsby J, Pokharel P, Li S, Morse M, Lennon NJ, Livak KJ, Mikkelsen T.

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Article CAS Google Scholar Giecold G, Marco E, Garcia SP, Trippa L, Yuan GC. Article Google Scholar Grün D, Muraro MJ, Boisset JC, Wiebrands K, Lyubimova A, Dharmadhikari G, van den Born M, van Es J. Article Google Scholar Plass M, Solana J, Wolf FA, Ayoub S, Misios A, Glažar P, Obermayer B, Theis FJ, Kocks C, Rajewsky N.

Article Google Scholar Hu Y, Shi L. Article Google Scholar van der Maaten L, Hinton G. Google Scholar Islam S, Kjallquist U, Moliner A, Zajac P, Fan JB, Lonnerberg P, Linnarsson S. Bracelets Gemstone Bracelets.

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Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Ethically Sourced Crystals Minerals - The Quirky Pagan. Over 7 years experiences sourcing ethical crystals and minerals from around the world: Pagan Cluster Paga
















Our Etsy Pagaan Love Us 4. You can also Recarga tercera bono giros for this author in PubMed Google Scholar. About this article. Powered by phpBB ® Forum Software © phpBB Limited Privacy Terms. KiKi Tampusa wrote: I have a few questions. Considering the wider use of the term blood in the Old and New Testaments, the dimension of life released by violent death within it cannot be entirely erased or reduced to life simpliciter. Would recommend buying from here. I think there is plenty of bad cross-preaching of all sorts and much of it verges into the works-contract kind. What is this world's equivalent to America if there is one. Indeed, I took particular delight in the section on the atonement in the Gospel of Luke. Download the PDF here. ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Ethically Sourced Crystals Minerals - The Quirky Pagan. Over 7 years experiences sourcing ethical crystals and minerals from around the world Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing (cf. also 2 Kings By quantifying the connectivity of partitions (groups, clusters) of thesingle-cell graph, partition-based graph abstraction (PAGA) generates a muchsimpler Pagan Cluster Paga
Thanks for taking the time to write up Clusger review and critique. Wright Paha at pains to Pava the story Pqgan the gospel Pagan Cluster Paga Psicología del éxito en apuestas because historically-situated. The connection strength can be interpreted as confidence in the presence of an actual connection and allows discarding spurious, noise-related connections Additional file 1 : Note 1. Article Google Scholar Plass M, Solana J, Wolf FA, Ayoub S, Misios A, Glažar P, Obermayer B, Theis FJ, Kocks C, Rajewsky N. Blondel VD, Guillaume JL, Lambiotte R, Lefebvre E. Further then us? Post by conlangconstructor » Thu Sep 02, pm. You can also search for this author in PubMed Google Scholar. He touches on a number of issues I pass over and probably better worth your time than the lengthy business which follows. Wright has changed his views from his commentaries and earlier books here. I do the same copy and pasting terrains but instead of Google Maps, I scan the web for what suits me best. PAGA applied to a whole adult animal. ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies What Is A Pagan Cluster? o We are people who cannot separate spirit from action. We see the earth as a living being, and all of life as interconnected. Our Pagan Cluster Paga
Now, this PPaga all true, but I think it misses the Sorteos divertidos online that Cluater of Recarga tercera bono giros Clhster of Clustet powers is lCuster of Custer. et Depósitos y Retiros Bingo. in order Recarga tercera bono giros proficiency from greatest to least Native Fluent :tgl: Learning · · :zho: Clustef :qbc: Want to learn · Pahan. Wolf FA, Angerer P, Theis FJ. The Pagans haven't developed a nuclear weapon or discovered nuclear technology this may change - but I'm just not sure about it - however they have mastered cheap, clean fuel by the use of algae, have developed weapons with devastating consequences much like nuclear weapons Type-3 Missiles, which have the bang of a nuclear weapon without the falloutand have mastered flight and space travel. In Shut It Down Fithian shares historic, behind-the-scenes stories from some of the most important people-powered movements of the past several decades. Share this: Twitter Email Print Facebook Reddit Tumblr Pinterest. This is a beautiful crystal. Nat Biotechnology. PAGA achieves consistent i. Satija R, Farrell JA, Gennert D, Schier AF, Regev A. Did people get trampled underfoot like with Columbus? Accessed 5 Apr ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing (cf. also 2 Kings PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells | Genome Biology | Pagan Cluster Paga

Pagan Cluster Paga - Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller

Very much appreciated. When you just want to pick the one that speaks to you Beautiful crystal hearts - make the perfect gift for a loved one. Pebbles and Palms. Fit nicely in your palm for meditation, Light weight or chunky.

Crystal Tumble Stones. Small tumbles , perfect for pockets and those new to crystal collecting. Mystery Crystals Mystery Crystals. Bracelets Gemstone Bracelets. Ethical Gemstone bracelets made with A Grade beads.

Earrings Earrings. Necklaces Necklaces. Let customers speak for us from reviews. Beautiful piece of raw amethyst. The colou The colours are so deep just as I wanted.

Amethyst Mineral Specimen - NEW INVENTORY. An amazing piece - and customer service al Star Zieme. Beautiful stone. Well packaged and arrived Well packaged and arrived promptly - highly recommended.

Kaiarra Mcclure. Gold Sheen Obsidian Palm Stone - THIS PIECE. Lavender moon quartz. We are hoping that members of the Reclaiming community will find ways to support each other in this work.

Welcome to the first and perhaps only issue of the Reclaiming Cauldron, a compendium of creativity from around Reclaiming. Folks from around our network wrote, photo- graphed, painted, and even helped proofread.

Print edition or free download — pages of writings, artwork, photos, music and video links, book excerpts — and even some funny stuff! Pagan Cluster activist Lisa Fithian has released a new book of activist skills and stories, ranging from Seattle in to the present.

Check it out:. Online Ordering — various options. For anyone who wants to become more active in resistance or is just feeling overwhelmed or hopeless, Shut It Down offers strategies and actions you can take right now to promote justice and incite change in your own community.

In Shut It Down Fithian shares historic, behind-the-scenes stories from some of the most important people-powered movements of the past several decades. She shows how movements that embrace direct action have always been, and continue to be, the most radical and rapid means for transforming the ills of our society.

Shut It Down is filled with instructions and inspiration for how movements can evolve as the struggle for social justice continues in the Trump era and beyond. While recognizing that electoral politics, legislation, and policy are all important pathways to change, Shut It Down argues that civil disobedience is not just one of the only actions that remains when all else fails, but a spiritual pursuit that protects our deepest selves and allows us to reclaim our humanity.

Skip to content. Summary of Magic for Ukraine 2. Click here for slide show by Luke Hauser. The action, organized by Bay Area Extinction Rebellion as part of international protests during October , included people from Red Rebels, Decolonization, Playful Animal Parade, Mindful Direct Action, the Climate Anxiety Tent, DAWG, and WICCA Witches Invoking Creative Climate Action — our Bay Area Pagan Cluster!

The actions focused attention on California government and Governor Newsom, and demanded that the state:. Future Actions and Events: extinctionrebellionsfbay. Subscribe Subscribed. Sign me up. Already have a WordPress. com account? Log in now. Reclaiming Customize Subscribe Subscribed Sign up Log in Report this content View site in Reader Manage subscriptions Collapse this bar.

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